Zombie Violet(TM) Fixable Viability Kit from BioLegend

Product Specs
ItemZombie Violet(TM) Fixable Viability Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423114
Quantity500 tests
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The 4-1BBζ costimulatory domain in chimeric antigen receptors enhances CD8+ T-cell functionality following T-cell receptor stimulationCancer Cell IntDecember 18, 2023Gerard J. Chu, Charles G. Bailey, Rajini Nagarajah, Sharon M. Sagnella, Stephen Adelstein, John E. J. Rasko[18]. Viability was assessed by staining with either Zombie Ultraviolet, Violet or Green (#423107, 423114 or 423111, Biolegend) at a 1:1000 dilution in PBS for 15 min at room temperature. Transduction efficiency

(2) Magnetic Resonance Imaging of Macrophage Response to Radiation TherapyCancers (Basel)December 17, 2023Harrison Yang, Brock Howerton, Logan Brown, Tadahide Izumi, Dennis Cheek, J. Anthony Brandon, Francesc Marti, Roberto Gedaly, Reuben Adatorwovor, Fanny Chapelinwells were harvested into flow cytometry tubes and rinsed in PBS prior to Zombie Violet (Cat# 423113/423114, Biolegend, San Diego, CA, USA) staining per manufacturer protocol. Cells were rinsed with Annexin-V

(3) CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cellsiScienceDecember 15, 2023Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, et al.either 7-AAD (Thermo-Fisher #A1310) diluted 1:1000 in the last washing step or Zombie Violet (BioLegend #423113) diluted 1:500 or 1:1000 in Ca/Mg-free DPBS in the very beginning of the procedure before stained with

(4) Targeted Polymersome Delivery of a Stapled Peptide for Drugging the Tumor Protein p53:BCL-2-Family Axis in Diffuse Large B-Cell LymphomaACS NanoDecember 12, 2023Mathew R. Schnorenberg, Katrina M. Hawley, Anika T. Thomas-Toth, Elyse A. Watkins, Yu Tian, Jeffrey M. Ting, Logan B. Leak, Isadora M. Kucera, Michal M. Raczy, Andrew L. Kung, et al.Invitrogen Fixable Blue Dead Cell Stain (L23105) or Zombie Violet Fixable Viability Kit (BioLegend 423113). Anti-Fab F(ab′)2 (Alexa Fluor 647 AffiniPure F(ab′)2 Fragment Donkey Anti-Mouse IgG (H+L); Jackson ImmunoResearch

(5) RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophagesbioRxivDecember 7, 2023Teodorus Theo Susanto, Victoria Hung, Andrew G. Levine, Craig H. Kerr, Yongjin Yoo, Yuxiang Chen, Juan A. Oses-Prieto, Lisa Fromm, Kotaro Fujii, Marius Wernig, et al.Subsequent cell pellets were resuspended in Zombie Violet Live-Dead Stain (1:500 in PBS; BioLegend, 423113) and incubated for 15 min in the dark. Cells were then washed with Cell Staining Buffer (0.1% NaN3,

(6) A flow-cytometry-based assay to assess the cytolytic activity against tumor cells by combination of mouse MAIT cells and natural killer cellsSTAR ProtocolsDecember 1, 2023Chie Sugimoto, Hiroyoshi Fujita, Hiroshi WakaoVybrant CFDA SE cell tracer kit,Thermo Fisher Scientific,Cat# V12883 Zombie Violet Flexible Viability kit,BioLegend,Cat# 423113 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Murine

(7) Tumor-derived biomarkers predict efficacy of B7H3 antibody-drug conjugate treatment in metastatic prostate cancer modelsJournal of Clinical InvestigationNovember 15, 2023Supreet Agarwal, Lei Fang, Kerry McGowen, JuanJuan Yin, Joel Bowman, Anson T. Ku, Aian Neil Alilin, Eva Corey, Martine P. Roudier, Lawrence D. True, et al.resulting single cells were resuspended in PBS and stained with 1:1,000 Zombie Violet dye (Biolegend, 423113) for 15 minutes to exclude dead cells. Cells were pelleted and resuspended in 100 μl staining buffer

(8) Epidermal growth factor receptor activation is essential for kidney fibrosis developmentNature CommunicationsNovember 14, 2023Shirong Cao, Yu Pan, Andrew S. Terker, Juan Pablo Arroyo Ornelas, Yinqiu Wang, Jiaqi Tang, Aolei Niu, Sarah Abu Kar, Mengdi Jiang, Wentian Luo, et al.analysis based on scatter signals and use of Zombie Violet™ Fixable Viability Kit (Biolegend, Cat# 423114). Isolation of kidney fibroblasts/myofibroblasts Kidney fibroblasts/myofibroblasts were isolated using

(9) Production of a p65fl/fl/LysMCre mouse model with dysfunctional NF-κB signaling in bone marrow-derived macrophagesInnate ImmunityNovember 1, 2023Ahmet K. Korkaya, Jeffrey Fischer, Anthony Peppers, Sean M. Crosson, Manira Rayamajhi, Edward A. Miao, Albert S. Baldwin, Jennifer W. BradfordLaboratory), for myeloid cell (CD11b+) and macrophage (CD11b+, F4/80+) populations. Zombie Violet (423113 Biolegend) was used as a viability dye. Antibody conjugations were as follows: CD11b-PE-Cyanine7 (25-0112-82

(10) Tumor-repopulating cell-derived microparticles elicit cascade amplification of chemotherapy-induced antitumor immunity to boost anti-PD-1 therapySignal Transduction and Targeted TherapyOctober 25, 2023Nana Bie, Tuying Yong, Zhaohan Wei, Qingle Liang, Xiaoqiong Zhang, Shiyu Li, Xin Li, Jianye Li, Lu Gan, Xiangliang Yangisolated tumor cells were stained with anti-Annexin V (catalog #640920) and Zombie Violet (catalog #423113) for apoptosis analysis. All antibodies applied for flow cytometry analysis were purchased from Biolegend

(11) Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulationiScienceOctober 20, 2023Kutaiba Alhaj Hussen, Emna Chabaane, Elisabeth Nelson, Shalva Lekiashvili, Samuel Diop, Seydou Keita, Bertrand Evrard, Aurélie Lardenois, Marc Delord, Els Verhoeyen, et al.RRID:AB_2737642 anti-human CD15 BV605,BD Biosciences,Cat# 562978562979; RRID:AB_2744292 Zombie Violet,BioLegend,Cat# 423113 ,, Biological samples,Biological samples,Biological samples ,, Umbilical cord blood,Unité de Thérapie

(12) Characterizing infection of B cells with wild-type and vaccine strains of measles virusiScienceOctober 20, 2023Logan Melot, Bettina Bankamp, Paul A. Rota, Melissa M. Coughlin562788), BV786 conjugated anti-CD38 (BD Horizon, 563964), and Zombie Violet viability stain (Biolegend, 423113). Positive fluorescent values were determined as signal above the isotype control for each fluorophore.

(13) Novel therapeutic strategies for injured endometrium: intrauterine transplantation of menstrual blood‑derived cells from infertile patientsStem Cell Research & TherapyOctober 15, 2023Satoshi Hosoya, Ryo Yokomizo, Harue Kishigami, Yukiko Fujiki, Erika Kaneko, Mitsuyoshi Amita, Takakazu Saito, Hiroshi Kishi, Haruhiko Sago, Aikou Okamoto, et al.centrifugation at 1000 rpm for 5 min, Zombie Violet™ Fixable Viability Kit (1:100, BioLegend, catalog number 423114) was used to remove dead cells for 20 min at room temperature according to the manufacturer’s protocol.

(14) Protocol for purification of cells in their native state using reversible aptamer-antidote pairsSTAR ProtocolsSeptember 1, 2023Martin D. Requena, Bethany Powell Gray, Bruce A. SullengerSolution (20nM),Thermo Fisher Scientific,Cat# 62249 Zombie Violet™ Fixable Viability Kit,BioLegend,Cat# 423113 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Target

(15) Multi-color Flow Cytometry Protocol to Characterize Myeloid Cells in Mouse Retina ResearchBIO-PROTOCOLAugust 20, 2023Wei Xiao, Rami A. Shahror, Carol A. Morris, Ruth B. Caldwell, Abdelrahman Y. Fouda(Sigma, catalog number: NC1179175)Zombie VioletTM Fixable Viability kit (BioLegend, catalog number: 423113)Jackson Immuno Research Labs normal rat serum (Fisher, catalog number: NC9834724)RBC lysis buffer for

(16) ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockadeNature communicationsJuly 10, 2023Liu X, Cen X, Wu R, Chen Z, Xie Y, Wang F, Shan B, Zeng L, Zhou J, Xie B, et al.HUABIO). The following antibodies for flow analysis were displayed: Zombie Violet™ Fixable Viability Kit (#423114; 1:200; Biolegend), PerCP/Cyanine5.5 anti-mouse CD45 (#103132; 1:200; Biolegend), PE/Cyanine7 anti-mouse CD3

(17) Primary Mouse Invariant Natural Killer T (iNKT) Cell Purification and TransductionBIO-PROTOCOLJuly 5, 2023Gloria Delfanti, Paolo Dellabona, Giulia Casorati(Santa Cruz, catalog number: SC-3598)Live/Dead Zombie Violet Fixable Viability kit (BioLegend, catalog number: 423114)Bovine serum albumin (BSA) fraction V (Roche, catalog number: 10735094001)EDTA stock solution (Cayman Chemical Company

(18) Transcriptome analysis of mesenchymal stromal cells of the large and small intestinal smooth muscle layers reveals a unique gastrontestinal stromal signatureBiochemistry and Biophysics ReportsJuly 1, 2023Takashi Chaen, Tamaki Kurosawa, Kazuhisa Kishi, Noriyuki Kaji, Madoka Ikemoto-Uezumi, Akiyoshi Uezumi, Masatoshi Horisingle-cell suspensions. Mononuclear cells were stained with the Zombie Violet Fixable Viability Kit (Cat# 423113; BioLegend, San Diego, CA, USA) for 15 min at 15–25 °C, followed by incubation with CD31-Alexa 488 conjugated

(19) Semaphorin 4B is an ADAM17-cleaved adipokine that inhibits adipocyte differentiation and thermogenesisMolecular metabolismJuly 1, 2023Amin A, Badenes M, Tüshaus J, de Carvalho É, Burbridge E, Faísca P, Trávníčková K, Barros A, Carobbio S, Domingos PM, et al.(1:100; 141717; Biolegend), Ly6C-APC-Cy7 (1:100; produced in-house), Zombie viability dye (1:1000; 423113; Thermo Fisher Scientific) and incubated for 20 min at 4 °C. The cells were afterwards wash in FACs

(20) A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein responseScience advancesJune 30, 2023Kar A, Jain D, Kumar S, Rajput K, Pal S, Rana K, Kar R, Jha SK, Medatwal N, Yavvari PS, et al.(#128006), Zombie Aqua fixable viability dye (#423102), and Zombie Violet Fixable Viability Kit (BV421) (#423113) were purchased from BioLegend, San Diego, USA. ATF4 (#11815), BAK (#33814S), BAX (#2772S), BCL-2 (#3498S),

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10/17/2019Zombie Violet Viability Dye Kabir KhanFollow the manufacturer's instructions to be able to stain live cells in flow cytometry for cells that need to be fixed and permeabilised to be able to stain intracellular proteins.

Zombie Violet(TM) Fixable Viability Kit from BioLegend

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Zombie Violet(TM) Fixable Viability Kit from BioLegend

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